Analysis of human histone H2AZ deposition in vivo argues against its direct role in epigenetic templating mechanisms.
Mol Cell Biol
; 26(14): 5325-35, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16809769
ABSTRACT
Chromatin is considered to be a principal carrier of epigenetic information due to the ability of alternative chromatin states to persist through generations of cell divisions and to spread on DNA. Replacement histone variants are novel candidates for epigenetic marking of chromatin. We developed a novel approach to analyze the chromatin environment of nucleosomes containing a particular replacement histone. We applied it to human H2AZ, one of the most studied alternative histones. We find that neither H2AZ itself nor other features of the H2AZ-containing nucleosome spread to the neighboring nucleosomes in vivo, arguing against a role for H2AZ as a self-perpetuating epigenetic mark.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Epigênese Genética
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article