Biological data warehousing system for identifying transcriptional regulatory sites from gene expressions of microarray data.
IEEE Trans Inf Technol Biomed
; 10(3): 550-8, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16871724
ABSTRACT
Identification of transcriptional regulatory sites plays an important role in the investigation of gene regulation. For this propose, we designed and implemented a data warehouse to integrate multiple heterogeneous biological data sources with data types such as text-file, XML, image, MySQL database model, and Oracle database model. The utility of the biological data warehouse in predicting transcriptional regulatory sites of coregulated genes was explored using a synexpression group derived from a microarray study. Both of the binding sites of known transcription factors and predicted over-represented (OR) oligonucleotides were demonstrated for the gene group. The potential biological roles of both known nucleotides and one OR nucleotide were demonstrated using bioassays. Therefore, the results from the wet-lab experiments reinforce the power and utility of the data warehouse as an approach to the genome-wide search for important transcription regulatory elements that are the key to many complex biological systems.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Armazenamento e Recuperação da Informação
/
Análise de Sequência de DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Bases de Dados Genéticas
/
Elementos Reguladores de Transcrição
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article