A new inhibitor of plasmin and trypsin from porcine leukocytes.
Biol Chem Hoppe Seyler
; 371(1): 57-61, 1990 Jan.
Article
em En
| MEDLINE
| ID: mdl-1691002
ABSTRACT
A new intracellular inhibitor of plasmin and trypsin was isolated from porcine leukocytes by ion exchange chromatography and affinity chromatography. In dodecyl sulphate gel electrophoresis a single protein band with an apparent molecular mass of 15 kDa was found under reducing conditions. On isoelectric focusing three protein bands with isoelectric points between pH 4.0 and 4.5 were found. The association rate constants and the inhibition constants were determined for porcine plasmin and bovine trypsin. The inhibitor shows no immunologic cross-reactivity with any of the tested leukocyte inhibitors. On the basis of its N-terminal amino-acid sequence a great degree of similarity to Kunitz-type inhibitors was observed.
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Base de dados:
MEDLINE
Assunto principal:
Inibidores da Tripsina
/
Fibrinolisina
/
Leucócitos
Limite:
Animals
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article