Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2.
J Clin Microbiol
; 44(9): 3443-7, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16954297
ABSTRACT
We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Líquido Cefalorraquidiano
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Reação em Cadeia da Polimerase
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Herpesvirus Humano 2
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Herpesvirus Humano 1
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Transferência Ressonante de Energia de Fluorescência
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Qualitative_research
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article