Enzymatic synthesis of L-pipecolic acid by Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida.
Biosci Biotechnol Biochem
; 70(9): 2296-8, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16960365
ABSTRACT
L-Pipecolic acid is a chiral pharmaceutical intermediate. An enzymatic system for the synthesis of L-pipecolic acid from L-lysine by commercial L-lysine alpha-oxidase from Trichoderma viride and an extract of recombinant Escherichia coli cells coexpressing Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida and glucose dehydrogenase from Bacillus subtilis is described. A laboratory-scale process provided 27 g/l of L-pipecolic acid in 99.7% e.e.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Pipecólicos
/
Microbiologia Industrial
/
Pseudomonas putida
/
Oxirredutases atuantes sobre Doadores de Grupo CH-NH
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article