Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit.
J Biol Chem
; 281(48): 36579-87, 2006 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-17015443
ABSTRACT
Nuclear export of the large ribosomal subunit requires the adapter protein Nmd3p to provide a leucine-rich nuclear export signal that is recognized by the export receptor Crm1. Nmd3p binds to the pre-60 S subunit in the nucleus. After export to the cytoplasm, the release of Nmd3p depends on the ribosomal protein Rpl10p and the GTPase Lsg1p. Here, we have carried out a mutational analysis of Nmd3 to better define the domains responsible for nucleocytoplasmic shuttling and ribosome binding. We show that mutations in two regions of Nmd3p affect 60 S binding, suggesting that its binding to the subunit is multivalent.
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Base de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Proteínas de Ligação a RNA
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article