Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients.
Mycopathologia
; 162(4): 273-80, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-17039273
ABSTRACT
Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.
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Base de dados:
MEDLINE
Assunto principal:
Tinha Versicolor
/
Dermatite Seborreica
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Malassezia
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article