Application of polyacrylamide gel electrophoresis/neutron activation analysis for protein quantification.
Biol Trace Elem Res
; 26-27: 85-91, 1990.
Article
em En
| MEDLINE
| ID: mdl-1704787
ABSTRACT
A combination of two methods, polyacrylamide gel electrophoresis (PAGE) and neutron activation analysis (NAA), has been applied to solutions containing phosphoproteins for the purpose of protein quantification. The proteins were separated by molecular weight using PAGE, and then the whole gel was activated by neutron bombardment. Densitometric measurements of the developed bands from 32P, taken from autoradiographs of the activated gels, resulted in quantification of the phosphorus, and then the related protein. This PAGE/NAA method was applied to several phosphoprotein-containing materials, including commercial milk products and reference materials, i.e., IAEA A-11, milk powder, and SRM 1845, Cholesterol in Egg Powder.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Eletroforese em Gel de Poliacrilamida
/
Análise de Ativação de Nêutrons
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article