Low-level laser therapy at different energy densities (0.1-2.0 J/cm2) and its effects on the capacity of human long-term cryopreserved peripheral blood progenitor cells for the growth of colony-forming units.
Photomed Laser Surg
; 24(5): 601-4, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-17069490
ABSTRACT
OBJECTIVE:
The aim of this research was to investigate the effects of low-level laser therapy (LLLT) at different energy densities (0.1-2.0 J/cm(2)) on the capacity of long-term cryopreserved peripheral blood progenitor cell (PBPC) for growth of colony-forming units (CFU) in vitro. BACKGROUND DATA There are no data concerning the effects of LLLT on human cryopreserved PBPC.METHODS:
Cryopreserved PBPC samples were thawed after 3 years in order to demonstrate the positive effect of LLLT and after 5 years in order to confirm the LLLT's proliferative effect. Cultures were plated in quadruplicate 35-mm-diameter Petri dishes in methylcellulose medium (2 x 10(5)/mL final concentration) and incubated for 14 days at 37 degrees C with 5% CO(2). A 685-nm diode laser with 25-mW optical power was used as the source of irradiation. Cultures were exposed to energy densities of 0.1, 0.5, 1.0, 1.5, and 2.0 J/cm(2) before incubation (10 irradiated and 10 controls at each energy density group).RESULTS:
A higher number of CFU was observed at the dose of 1.0 J/cm(2) (control 21.3 +/- 8.5 x 10(5) cells, irradiated 40.1 +/- 10.5 x 10(5) cells, p < 0.001). No differences were observed in cultures exposed to doses of 0.1, 0.5, and 1.5 J/cm(2). A decreased number of CFU was demonstrated in samples exposed to the dose of 2.0 J/cm(2) (control 21.4 +/- 11.9 x 10(5) cells, p = 0.013). PBPC samples cryopreserved for 5 years were thawed for CFU assays and exposed to a single dose of 1.0 J/cm(2); once again the exposed group showed a higher number of CFU (control 8.8 +/- 7.8 x 10(5) cells, irradiated 18.1 +/- 13.1 x 10(5) cells, p = 0.026).CONCLUSION:
Dependent upon the energy density, LLLT elevates (1.0 J/cm(2)) or decreases (2.0 J/cm(2)) the potential of long-term cryopreserved PBPC for growth of CFU in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Criopreservação
/
Terapia com Luz de Baixa Intensidade
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article