Gene transfer in humans using a conditionally replicating lentiviral vector.
Proc Natl Acad Sci U S A
; 103(46): 17372-7, 2006 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-17090675
ABSTRACT
We report findings from a clinical evaluation of lentiviral vectors in a phase I open-label nonrandomized clinical trial for HIV. This trial evaluated the safety of a conditionally replicating HIV-1-derived vector expressing an antisense gene against the HIV envelope. Five subjects with chronic HIV infection who had failed to respond to at least two antiviral regimens were enrolled. A single i.v. infusion of gene-modified autologous CD4 T cells was well tolerated in all patients. Viral loads were stable, and one subject exhibited a sustained decrease in viral load. CD4 counts remained steady or increased in four subjects, and sustained gene transfer was observed. Self-limiting mobilization of the vector was observed in four of five patients. There is no evidence for insertional mutagenesis after 21-36 months of observation. Immune function improved in four subjects. Lentiviral vectors appear promising for gene transfer to humans.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
Lentivirus
/
Vetores Genéticos
Limite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article