Your browser doesn't support javascript.
loading
Identification of B- and T-cell epitopes within the MTP40 protein of Mycobacterium tuberculosis and their correlation with the disease course.
Falla, J C; Parra, C A; Mendoza, M; Franco, L C; Guzmán, F; Forero, J; Orozco, O; Patarroyo, M E.
Afiliação
  • Falla JC; Instituto de Inmunología, Hospital San Juan de Dios, Bogotá, Colombia.
Infect Immun ; 59(7): 2265-73, 1991 Jul.
Article em En | MEDLINE | ID: mdl-1711013
Synthetic peptides derived from the amino acid sequence of MTP40, a recently characterized Mycobacterium tuberculosis protein, were tested by two different immunological assays in 91 individuals. For the purposes of this study, the population was distributed in four groups: active tuberculosis (TBC) patients with elevated bacillus loads (BK+), active TBC patients with low bacillus loads (BK-), healthy individuals living in the same household with tuberculous patients (HH), and normal individuals, who had presumably never been in contact with the bacilli (control). We found that T cells of individuals belonging to the HH group showed the highest and most frequent recognition of these peptides in a T-cell proliferation assay, while their antibodies showed the lowest recognition of these peptides when tested by enzyme-linked immunosorbent assay. In contrast, TBC patients revealed an inverse pattern of immune response. Interestingly, one of these peptides (P7) was recognized by T cells of 64% of the HH individuals and by 4.5% of normal donors. Another peptide (P4) was recognized by 55% of sera from BK+ patients and by 5.5% of normal donors. The results presented here indicate the existence of T- and B-cell epitopes within the MTP40 protein. Given the particular recognition pattern of this protein, added to the fact that it appears to be a species-specific antigen of M. tuberculosis, a detailed study of the immune response to it may be useful in the design of more accurate diagnostic tests and an improved vaccine against human TBC.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfolipases Tipo C / Proteínas de Bactérias / Linfócitos B / Linfócitos T / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 1991 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfolipases Tipo C / Proteínas de Bactérias / Linfócitos B / Linfócitos T / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 1991 Tipo de documento: Article