Strain typing and determination of population structure of Candida krusei by multilocus sequence typing.
J Clin Microbiol
; 45(2): 317-23, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17122025
ABSTRACT
A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C. krusei is diploid for at least part of its genome. The C. krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei clinical isolates. MLST data for 122 independent C. krusei isolates from a range of geographical sources were analyzed by eBURST, structure, and the unweighted-pair group method using average linkages to derive a population structure comprising four subtype strain clusters. There was no evidence of geographical associations with particular subtypes. Data for pairs of isolates from seven patients showed that each patient was colonized and/or infected with strain types that were indistinguishable by MLST. The C. krusei MLST database can be accessed online at http//pubmlst.org/ckrusei/.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Candida
/
Proteínas Fúngicas
/
Técnicas de Tipagem Micológica
/
Análise de Sequência de DNA
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article