Structural basis for ribosome recruitment and manipulation by a viral IRES RNA.
Science
; 314(5804): 1450-4, 2006 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-17124290
ABSTRACT
Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Vírus de RNA
/
Biossíntese de Proteínas
/
RNA Viral
/
Sequências Reguladoras de Ácido Ribonucleico
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article