Identification of peroxisome-proliferator responsive element in the mouse HSL gene.
Biochem Biophys Res Commun
; 352(2): 526-31, 2007 Jan 12.
Article
em En
| MEDLINE
| ID: mdl-17134676
ABSTRACT
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5'-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPARgamma) and retinoid X receptor (RXRalpha) interacted with the region. Binding of the PPARgamma/RXRalpha heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPARgamma/RXRalpha heterodimer, and suggest that a cis-acting element regulates the HSL gene expression.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Esterol Esterase
/
Regiões Promotoras Genéticas
/
Adipócitos
/
Proliferadores de Peroxissomos
/
Elementos de Resposta
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article