T-cell inhibition does not aggravate bacterial translocation from rat small bowel.
Transpl Immunol
; 16(3-4): 208-14, 2006 Nov.
Article
em En
| MEDLINE
| ID: mdl-17138055
ABSTRACT
BACKGROUND:
T-cell mediated immunity has been proposed to have an important function in the defence against translocating microbes from the gastrointestinal tract. After small bowel transplantation massive T-cell immunosuppression is necessary to avoid rejection. As a consequence, infections with intestinal bacteria are the main contributors to mortality in this setting. This could further imply that T cells are important in limiting bacterial translocation. In a model for bacterial translocation from small bowel in the rat we examined the outcome of T-cell inactivation.METHODS:
The studies were performed in a model of bacterial translocation from a Thiry-Vella loop of small bowel in the rat. The animals were treated with an anti-alpha/beta T-cell receptor monoclonal antibody (R73). Inhibition of T-cell activation was also made using the immunosuppressive drug cyclosporin A. All animals were sacrificed on day 3 postoperatively and translocation to the mesenteric lymph nodes, liver, spleen, lung and blood was evaluated.RESULTS:
Treatment with R73 resulted in an almost complete labelling of T cells but did not result in any increased bacterial translocation compared to animals treated with saline. Neither did immunosuppression with cyclosporin A.CONCLUSIONS:
In the model of bacterial translocation from a defunctionalised loop of small bowel the inhibition of T cells does not increase bacterial translocation to mesenteric lymph nodes or promote the systemic spread of the translocating bacteria. This indicates that T cells do not have any important protective function against translocating microbes from defunctionalised small bowel.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Translocação Bacteriana
/
Intestino Delgado
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article