Quantitative micro positron emission tomography (PET) imaging for the in vivo determination of pancreatic islet graft survival.
Nat Med
; 12(12): 1423-8, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17143277
ABSTRACT
Islet transplantation is an attractive approach for treating type-1 diabetes, but there is a massive loss of transplanted islets. It is currently only possible to estimate islet mass indirectly, through measurement of circulating C-peptide and insulin levels. This type of estimation, however, is not sufficiently sensitive or reproducible for follow-up of individuals who have undergone islet transplantation. Here we show that islet graft survival could be assessed for 1 month in diabetic NOD mice using 9-(4-[(18)F]-fluoro-3-hydroxymethylbutyl)guanine ([(18)F]FHBG)-positron emission tomography (PET) technology, the PET signal reflecting insulin secretory capacity of transplanted islets. Expression of the gene encoding viral interleukin-10 (vIL-10), was measurable in real time with PET scanning. Additionally, we addressed the clinical potential of this approach by visualizing transplanted islets in the liver, the preferred clinical transplantation site. We conclude that quantitative in vivo PET imaging is a valid method for facilitating the development of protocols for prolonging islet survival, with the potential for tracking human transplants.
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Base de dados:
MEDLINE
Assunto principal:
Transplante das Ilhotas Pancreáticas
/
Tomografia por Emissão de Pósitrons
/
Sobrevivência de Enxerto
Tipo de estudo:
Evaluation_studies
/
Guideline
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article