Chemical behavior of bis(3'-5')diguanylic acid in aqueous solutions.

ABSTRACT

This paper describes unique behavior of bis(3'-5')diguanylic acid (c-di-GMP) under some conditions. Thus, c-di-GMP exists as the monomer in aprotic organic solvents such as DMSO. By contrast, c-di-GMP smoothly aggregates in water and in low-concentration aqueous solutions of some salts, such as sodium chloride and ammonium acetate, to give a mixture of many aggregates. The resulting multiple aggregates converge to the single compound (provably the monomer) in a >154 mM (0.9%) sodium chloride aqueous solution, in a >100 mM ammonium acetate buffer, and in a >100 mM phosphate buffer.