Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation.
In Vitro Cell Dev Biol Anim
; 42(8-9): 263-72, 2006.
Article
em En
| MEDLINE
| ID: mdl-17163780
ABSTRACT
A continuous cell line, PBLE, was developed from the adherent cells in a culture of peripheral blood leukocytes from the American eel, Anguilla rostrata. The cells were grown in Leibovitz's L-15 basal medium supplemented with 20% fetal bovine serum (FBS). Under normal culture conditions at 18 degrees C, the morphology of PBLE was fibroblast-like. The cultures have been subcultured over 80 times and have been cryopreserved successfully. These cells have a diploid karyotype of 38 chromosomes, survived temperatures from 5 to 36 degrees C, and proliferated at temperatures from 5 degrees C to at least 30 degrees C. PBLE underwent apoptosis in response to gliotoxin, but did not show a respiratory burst. Results suggest that PBLE may have arisen from a circulating mesenchymal stem cell. PBLE was susceptible to Chum salmon reovirus (CSV) and supported CSV replication. Therefore this cell line should be useful in studying eel specific virus-host interactions.
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Base de dados:
MEDLINE
Assunto principal:
Linhagem Celular
/
Técnicas de Cultura de Células
/
Leucócitos
/
Anguilla
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article