[Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture].
Zhongguo Zhong Yao Za Zhi
; 31(19): 1580-3, 2006 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-17165578
ABSTRACT
OBJECTIVE:
To illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.METHOD:
High performance liquid chromatography and plant tissue culture were applied. RESULT ANDCONCLUSION:
Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Sacarose
/
Pueraria
/
Proliferação de Células
/
Isoflavonas
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article