Phosphorylated vasodilator-stimulated phosphoprotein is localized on mitotic spindles of the gastric cancer cell line SGC-7901.
World J Gastroenterol
; 12(46): 7478-81, 2006 Dec 14.
Article
em En
| MEDLINE
| ID: mdl-17167837
ABSTRACT
AIM:
To elucidate the localization of vasodilator stimulated phosphoprotein (VASP), a cytoskeletal organizing protein and a substrate of protein kinases A and G in mitotic gastric cancer cells.METHODS:
Immunofluorescence microscopy was used to observe the localization of alpha-tubulin, VASP and Ser157 phosphorylated VASP (p-VASP) in interphase of mitotic gastric cancer of the cell line SGC-7901.RESULTS:
Immunofluorescence staining showed that p-VASP but not VASP was co-localized with alpha-tubulin on spindle poles and fibers in prophase, metaphase and anaphase of the mitotic process of the gastric cancer cell line SGC-7901. H89, an inhibitor of protein kinases A and G, had no effect on the localization of p-VASP on the spindles.CONCLUSION:
VASP may play a role in assembling and stabilizing the mitotic spindle of cells, and phosphorylation of the protein is the precondition for it to exert this function.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Neoplasias Gástricas
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Moléculas de Adesão Celular
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Proteínas dos Microfilamentos
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Fuso Acromático
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article