Effect of HbS in the determination of HbA(2) with the TOSOH HLC-723G7 analyzer and the HELENA Beta-Thal Quik column kit.
Clin Biochem
; 40(3-4): 242-7, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17196577
ABSTRACT
OBJECTIVES:
The analytical performance of the TOSOH HLC-723G7 hemoglobin HPLC analyzer and the effect of the presence of HbS in the determination of HbA(2) using HPLC and manual column methods. DESIGN ANDMETHODS:
The performance characteristics of the TOSOH HLC-723G7 analyzer in the determination of HbA(2) were compared to those of the HELENA Beta-Thal Quik column. The effect of HbS presence in the samples was quantified using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method.RESULTS:
Within-run and between-run CVs for HbA(2) were better for the TOSOH HPLC analyzer than for the HELENA manual column method. The presence of HbS in the samples produces a strong positive bias in the %HbA(2) values when using both the HPLC and manual column methods, compared to the alkaline electrophoresis gel.CONCLUSION:
Both the TOSOH HPLC and the manual column are reliable methods for %HbA(2) determination when no HbS is detectable in the samples. When HbS is present, the gel electrophoresis method gives more accurate results.
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Base de dados:
MEDLINE
Assunto principal:
Hemoglobina A2
/
Hemoglobina Falciforme
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia por Troca Iônica
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article