Screening and mutagenesis of a novel Bacillus pumilus strain producing alkaline protease for dehairing.
Lett Appl Microbiol
; 44(1): 1-6, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17209806
ABSTRACT
AIMS:
To characterize and optimize a novel Bacillus pumilus strain isolated from biological waste which produces protease with excellent dehairing effect. This newly isolated strain could be utilized in the industrial leather dehairing process. METHODS ANDRESULTS:
Bacterial strains secreting proteases were screened from biological wastes. Positive clones were further characterized by analysing their efficacy in dehairing and effects on collagen integrity. Among 171 colonies tested, a strain BA06, identified as B. pumilus, was picked owing to its efficient dehairing capabilities with minimal impact on collagen. By combined mutagenesis using UV, N-methyl-N'-nitro-N-nitrosdguanidine and Co(60)-gamma-rays, this strain was further improved with regard to its alkaline protease production. The alkaline protease activity of the mutant strain SCU11was greatly improved up to 6000 U ml(-1), in comparison with its parent strain BA06 of 1200 U ml(-1).CONCLUSIONS:
By using screening and mutagenesis methods, we have successfully created a B. pumilus strain that can produce high levels of alkaline proteases that are able to efficiently remove hair from skin with minimal damage on the collagen. SIGNIFICANCE AND IMPACT OF THE STUDY This strain could be used in commercial alkaline protease production for leather dehairing.
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Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Curtume
/
Bacillus
/
Proteínas de Bactérias
/
Cabelo
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article