Construction of a dimethyl sulfoxide sensor based on dimethyl sulfoxide reductase immobilized on a au film electrode.
Anal Sci
; 23(1): 55-8, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17213624
ABSTRACT
A novel dimethyl sulfoxide (DMSO) sensor using DMSO reductase and film electrodes was constructed. The Au and Ag electrodes were fabricated on slide glass by vacuum deposition and the application of a photolithographic technique. The micro-chamber (4 x 50 x 1 mm, volume 200 microl) was fabricated on a poly(dimethylsiloxane) (PDMS) polymer. The Pt electrode was implanted in a PDMS polymer. DMSO reductase was immobilized on a Au film electrode with bovine serum albumin (BSA)-glutaraldehyde. This sensor could determine DMSO in an unpurged aqueous solution with glucose oxidase (GOD) and catalase (CAT) for oxygen removal. The DMSO sensor showed a linear response within 1 mM DMSO with a correlation coefficient of 0.999. The detection limit was 200 microM (3sigma), and the sensitivity was 23.8 mA M(-1) cm(-2). The relative standard deviations at each concentration were within 3.6%.
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Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Dimetil Sulfóxido
/
Enzimas Imobilizadas
/
Proteínas Ferro-Enxofre
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article