Metabolic and structural effects of HIV infection in human peripheral blood mononuclear cells can be monitored with 1H NMR spectroscopy.
J Acquir Immune Defic Syndr (1988)
; 4(11): 1073-81, 1991.
Article
em En
| MEDLINE
| ID: mdl-1721652
ABSTRACT
Infection of human peripheral blood lymphocytes by human immunodeficiency virus type 1 (HIV-1) was investigated by means of 1H nuclear magnetic resonance spectroscopy, taking advantage of the presence of signals from fluid lipid domains in the membrane of stimulated lymphocytes. A transient decrease of the lipid methylene signal intensity was observed at the time of HIV internalization, monitoring a general rearrangement of membrane structure associated with virus entry. A similar effect was also observed a few days after infection, when HIV particles are released by infected cells as demonstrated by high reverse transcriptase activity in cell supernatant. Signals arising from choline-based metabolites were also affected by HIV infection, indicating a possible slowing down of phospholipid synthesis.
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Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Espectroscopia de Ressonância Magnética
/
Infecções por HIV
Limite:
Humans
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article