[Morphologic HIV demonstration in formalin embedded material--techniques, problems, results]. / Morphologischer HIV-Nachweis an formalinfixiertem Material--Techniken, Probleme, Ergebnisse.
Verh Dtsch Ges Pathol
; 75: 149-54, 1991.
Article
em De
| MEDLINE
| ID: mdl-1724819
ABSTRACT
Formalin-fixed, paraffin-embedded material of archival specimens is suitable for a morphological HIV-detection Infected cells with HIV in the proliferative phase can be demonstrated with reliable results on tissue sections by immunohistological technics using new antibodies. In situ nucleic acid hybridisation technics can also show HIV in the expression phase on paraffin-embedded material, but often fail in demonstrating latently HIV-infected cells. The DNA-Polymerase chain reaction can detect latent Provirus in morphologically defined areas of paraffin sections even in autopsy material, i.e. lymphnodes and even eyes of patients with HIV-Infection, but requires precaution and control with respect to contamination.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome da Imunodeficiência Adquirida
/
HIV
Limite:
Humans
Idioma:
De
Ano de publicação:
1991
Tipo de documento:
Article