[Importance of PCR for the diagnostics of canine babesiosis]. / Bedeutung der PCR in der Diagnostik der caninen Babesiose.
Schweiz Arch Tierheilkd
; 148(12): 633-40, 2006 Dec.
Article
em De
| MEDLINE
| ID: mdl-17263080
ABSTRACT
Clinical standards to confirm babesiosis in dogs include the direct identification of the infectious agent in blood smears and serological assays for Babesia canis-specific antibodies. Here, we demonstrate in seven cases (with data on anamnesis, clinics, laboratory diagnostics, and therapeutic outcomes) that a new diagnostic procedure is required. This is the molecular-genetic identification of babesia by real time PCR allowing an unequivocal identification of the infectious agents. Indeed, all seven patients presenting severe clinical symptoms were PCR-positive, but only two of them had specific antibodies and showed babesia in their bloodstream. Six of the dogs appeared to have acquired babesiosis while travelling abroad, and one in the Swiss canton of Schaffhausen.
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Base de dados:
MEDLINE
Assunto principal:
Babesia
/
Babesiose
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Doenças do Cão
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Limite:
Animals
Idioma:
De
Ano de publicação:
2006
Tipo de documento:
Article