Protection of mice against rotavirus challenge following intradermal DNA immunization by Biojector needle-free injection.
Vaccine
; 25(16): 3215-8, 2007 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-17280754
ABSTRACT
Mucosal administration (intranasal or oral) of a VP6 rotavirus vaccine to mice consistently elicits high levels of protection after rotavirus challenge (93->99% reductions in fecal rotavirus shedding) but only when co-administered with an effective adjuvant such as LT(R192G). Here, we showed that Biojector needle-free injection of VP6-encoded plasmids also induced protection (85-93%) when they were co-administrated with LT(R192G)-encoded plasmids. A reduction in the amount of VP6 plasmid from 50 to 10 microg reduced protection from 93 to 70%, but the immunized mice remained significantly (P<0.05) protected. Intramuscular needle injection of VP6/LT(R192G)-plasmids also induced significant protection (66%).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
DNA Viral
/
Vacinas contra Rotavirus
/
Proteínas do Capsídeo
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article