[The clinical application of spectral karyotyping in the analysis of chromosomal abnormalities].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 24(1): 80-3, 2007 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-17285551
ABSTRACT
OBJECTIVE:
To evaluate value of spectral karyotyping (SKY) in the detection of chromosomal abnormalities.METHODS:
A total of 17 metaphase chromosome samples were investigated by SKY, including 10 normal and 5 balanced translocation samples of peripheral blood lymphocytes, one der(Y) sample of peripheral blood lymphocytes and one marker chromosome sample of amniotic fluid cells. The results were compared with those of G-banding diagnosis.RESULTS:
Ten normal and 5 balanced translocation samples were diagnosed successfully by SKY in accordance with the results of G-banding; furthermore, SKY analysis revealed that the der(Y) fragment originated from p-arm of chromosome 21 while the marker chromosome originated from chromosome 5.CONCLUSION:
SKY is a very sensitive and specific whole genome analysis tool for chromosomal abnormality diagnosis, and exceedingly valuable in the diagnosis on complex chromosomal abnormalities that can not be determined by G-banding.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Aberrações Cromossômicas
/
Cariotipagem Espectral
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article