[Study on rat's DNA damage of p53 induced by chlorinated acetic acids of drinking water disinfection by-products].

ABSTRACT

OBJECTIVE: To study on the DNA damage of p53 induced by dichloroacetic acid(DCA) and trichloroacetic acid( TCA), approve their genotoxicity and discuss molecular mechanism of their carcinogenic action. METHODS: Administered SD rats with DCA or TCA by i.p. injection, extracted DNA from rat's liver, and then used RDPCR to detect DNA damage of exon 70f p53 gene. RESULTS: Two hybridization bands were detected in treated group induced by DCA. It was indicated that DCA can result in DNA damage of exon 7 of p53 gene of rat's liver tissue, and there were two broken sites. It was not detected damage of exon 7 of p53 gene of rat induced by TCA. CONCLUSION: There may be the relationship between DCA carcinogenic action and the damage of p53. The result that the damage of p53 of target tissue detected by RDPCR was consistent well with rat carcinogenic test.