The function of DNA polymerases in DNA repair synthesis of ultraviolet-irradiated human fibroblasts.
Biochim Biophys Acta
; 1129(2): 155-60, 1992 Jan 06.
Article
em En
| MEDLINE
| ID: mdl-1730053
ABSTRACT
Ultraviolet-induced DNA repair synthesis was measured in saponin-permeabilized normal human fibroblasts by the incorporation of [alpha-32P]dTMP into DNA. The involvement of DNA polymerases alpha, beta, delta, and epsilon in excision repair of pyrimidine dimers was examined using specific inhibitors. Dose-response curves resulting from experiments with up to 12 different inhibitor concentrations were analyzed by linear regression. Inhibitor concentrations at which repair activity was reduced to 50% were calculated. The following K50 values were found aphidicolin, 0.2 microM; ddTTP, 12.5 microM; butylphenyl-dGTP, 7.6 microM; butylanilino-dATP, 6.0 microM. Comparison of K50 values with in vitro Ki values of DNA polymerases revealed that in permeabilized human fibroblasts reparative DNA synthesis is catalyzed by DNA polymerase delta and by DNA polymerase epsilon.
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Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
DNA Polimerase Dirigida por DNA
/
Reparo do DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article