Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
FEBS Lett
; 581(7): 1275-86, 2007 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-17359980
ABSTRACT
The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coronavirus
/
Vírus da Bronquite Infecciosa
/
Ciclina D1
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article