DNA-directed assembly of supramolecular fluorescent protein energy transfer systems.
Bioconjug Chem
; 18(3): 621-7, 2007.
Article
em En
| MEDLINE
| ID: mdl-17378598
ABSTRACT
Fluorescent proteins with a wide variety of physicochemical properties have evolved in the past few years. The use of these proteins for applications in biomolecular nanosciences requires their precise positioning at the nanometer length scale. To address this challenge, we report here on the self-organization of DNA-tagged fluorescent probes to construct a set of photofunctional supramolecular complexes which include the enhanced yellow fluorescent protein (EYFP). The optical functionality is based on the strongly distance dependent fluorescence resonance energy transfer (FRET), occurring between the donor (EYFP) and an acceptor fluorophore, i.e., the fluorescent dye Atto647. The photophysical properties of four bimolecular FRET complexes, each possessing a well-defined donor-acceptor distance defined by the length of the interconnecting DNA backbone, are investigated by two-dimensional photoluminescence excitation spectroscopy (2D-PLE).
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Nanotecnologia
/
Nanopartículas
/
Corantes Fluorescentes
/
Proteínas Luminescentes
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article