Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells.
Microbiology (Reading)
; 153(Pt 4): 1059-1069, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17379714
ABSTRACT
Salmonella enterica serovar Typhimurium can be internalized by immature dendritic cells (DCs). The interacting host and bacterial molecules initiating this process remain uncharacterized. The objective of this study was to investigate whether specific fimbriae are involved in the early step of binding and uptake of Salmonella by DCs. Type 1 fimbriated S. enterica serovar Typhimurium or recombinant Escherichia coli expressing the type 1 fimbriae showed a significantly greater ability to attach to murine bone-marrow-derived DCs than non-fimbriated bacteria. The FimH adhesin was required for efficient interactions with DCs, since fimbriated fimH mutants were impaired in both binding and internalization. Finally, the internalization involved a FimH-dependent process but did not require sipB, a gene essential for Salmonella-mediated invasion of mammalian epithelial cells. Collectively, these data suggest that the bacterial interaction of DCs through the type 1 fimbrial adhesin FimH is sufficient to target S. enterica serovar Typhimurium for cellular uptake.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
Aderência Bacteriana
/
Células Dendríticas
/
Adesinas Bacterianas
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article