Anti-BDCA-4 (neuropilin-1) antibody can suppress virus-induced IFN-alpha production of plasmacytoid dendritic cells.
Immunol Cell Biol
; 85(5): 383-90, 2007 Jul.
Article
em En
| MEDLINE
| ID: mdl-17404592
ABSTRACT
Plasmacytoid dendritic cells (PDC) in human blood are the main source of virus-induced interferon (IFN)-alpha. They exhibit a lineage-negative phenotype but all express BDCA-4, which is homologous to the neuronal receptor neuropilin-1. Specific staining with anti-BDCA-4 antibody is used for positive isolation of PDC from blood by magnetic cells sorting. Here, it is demonstrated that these positively selected PDC showed reduced or completely abolished IFN-alpha release compared to unstained PDC, which were negatively selected by magnetic depletion of lineage-positive blood mononuclear cells. In addition, treatment of these unstained PDC with anti-BDCA-4 mAb also resulted in at least two-fold lower or reduced virus-induced IFN-alpha production. It is shown that the antibody not only affects cell survival or block virus attachment but also reduces IFN-alpha release induced by non-viral CpG oligodeoxynucleotides. In conclusion, data suggest an immunoregulatory role for BDCA-4 on PDC as demonstrated for IFN-alpha response to virus.
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Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Interferon gama
/
Neuropilina-1
/
Vírus do Sarcoma de Rous
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article