Preparation and characterization of hydroxyapatite/collagen nanocomposite gel.

ABSTRACT

The self-organized hydroxyapatite/colagen (HAp/Col) nanocomposite fiber (79.6/20.4 weight ratio) was synthesized by a co-precipitation method using Ca(OH)2, H3PO4, and Col as starting substances. The gelation of the nanocomposite is essential in the application of the scaffold for bone tissue engineering. We successfully prepared HAp/Col nanocomposite gels by a facile novel method using a sodium phosphate buffer at pH 6.8. The water-insoluble nanocomposite was homogeneously dispersed in the buffer to form a viscous mixture, and gels were obtained after incubating of the mixture at 37 degrees C. The mechanical strength of the gels was analyzed against the incubation time. The demineralized gel with EDTA had the typical nanostructure of native type I Col fibers from the results of scanning electron microscopy (SEM) and atomic force microscopy (AFM); the dense network of type I Col nano-fibers below 100 nm in diameter, and the periodic pattern of 68.8+/-4.4 nm (mean +/- SD) along the fibers were observed. The gelation of the HAp/Col nanocomposite in the buffer is attributed to the physical cross-linking through entanglement of the reconstituted Col fibrils.