ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na+ entry.
Biochem Biophys Res Commun
; 357(4): 1177-82, 2007 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-17466270
ABSTRACT
Reversal of the plasma membrane Na(+)/Ca(2+) exchanger (NCX) has been shown to mediate Ca(2+) influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our laboratory to mediate Ca(2+) influx in rat aortic smooth muscle cells (RASMCs) following ATP stimulation. In this communication, we provide further detail of this functional coupling by indirectly measuring NCX reversal. We found that NCX reversal, induced by the removal of extracellular Na(+), was increased following stimulation with ATP and the diacylglycerol analog 1-Oleoyl-2-acetyl-sn-glycerol. This increased NCX reversal was attenuated by SKF-96365, an inhibitor of non-selective cation channels, and by activation of protein kinase C with phorbol ester 12-tetradecanoylphorbol-13 acetate. These data are consistent with the known properties of TRPC6 and further support that functional coupling of TRPC6 and NCX occurs via a receptor-operated, rather than store-operated, cascade in RASMCs.
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Base de dados:
MEDLINE
Assunto principal:
Aorta
/
Sódio
/
Trifosfato de Adenosina
/
Cálcio
/
Trocador de Sódio e Cálcio
/
Miócitos de Músculo Liso
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article