PIM-2 is an independent regulator of chondrocyte survival and autophagy in the epiphyseal growth plate.
J Cell Physiol
; 213(1): 246-51, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17476689
ABSTRACT
The overall goal of the investigation was to examine the activity and role of the PIM serine/threonine protein kinases in the growth plate. We showed for the first time that PIM-2 was highly expressed in epiphyseal chondrocytes and that the kinase was required for critical activities linked to cell survival. These activities were independent of those mediated by Akt-1. It was noted that PIM-2 protected chondrocytes from rapamycin sensitized (TOR inhibited) cell death. Since inhibition of mTOR caused autophagy, we examined the autophagic response of PIM-2 silenced cells. We showed that PIM-2 promoted expression and organization of autophagic proteins LC3, and Beclin-1 and enhanced lysosomal acidification. At the same time, PIM-2 modulated the activity of a key regulator of apoptosis, BAD. Since BAD inhibition and Beclin-1 expression activated autophagy, it is likely that induction of the autophagic pathway would serve to inhibit apoptosis and preserve the life of the terminally differentiated chondrocyte. We conclude that PIM-2 regulates a new intermediate stage in the differentiation pathway, the induction of autophagy.
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Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Proteínas Proto-Oncogênicas
/
Proteínas Serina-Treonina Quinases
/
Condrócitos
/
Lâmina de Crescimento
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article