A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose.
Biosens Bioelectron
; 23(1): 135-9, 2007 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-17478087
ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 microA/mA cm2. The low detection limit was estimated to be 20 microM (S/N=3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Óxido de Zinco
/
Técnicas Biossensoriais
/
Cobalto
/
Nanoestruturas
/
Eletroquímica
/
Glucose
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article