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Effects of polyphenols derived from fruit of Crataegus pinnatifida on cell transformation, dermal edema and skin tumor formation by phorbol ester application.
Kao, Erl-Shyh; Wang, Chau-Jong; Lin, Wea-Lung; Chu, Chia-Yih; Tseng, Tsui-Hwa.
Afiliação
  • Kao ES; Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Food Chem Toxicol ; 45(10): 1795-804, 2007 Oct.
Article em En | MEDLINE | ID: mdl-17493734
ABSTRACT
The dried fruits of Crataegus pinnatifida have been used traditionally as oriental medicine and local soft drink material recently. Previously, we demonstrated that C. pinnatifida exhibited anti-oxidation and anti-inflammatory potential. To clarify the active components in anti-transformation and anti-tumor promotion, we collected the polyphenol fraction (CF-TP) of hot-water extracts from dried fruits of C. pinnatifida for the following study. By anchorage-independent transformation assay, CF-TP significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in JB6 P(+) cells. Moreover, we found that CF-TP inhibited the expression of osteopontin (OPN), a transformational marker, and the activation of NF-kappaB and AP-1 induced by TPA in JB6 P(+) cells. In addition, we evaluated the effect of CF-TP on TPA application to ICR mouse skin with measurement of H(2)O(2) production, myeloperoxidase (MPO) activity, edema formation, epidermal thickness and leukocyte infiltration. As a result, CF-TP significantly inhibited the generation of reactive oxygen species (ROS) and the phenomena of inflammation induced by TPA. It also suppressed the expression of COX-2 and iNOS, and the activation of ornithine decarboxylase (ODC). Furthermore, CF-TP inhibited benzo[a]pyrene (B[a]P)/TPA-induced skin tumor formation and decreased the incidence of tumor. These results indicate that CF-TP possesses potential as a cancer chemopreventive agent against tumor promotion.
Assuntos
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Base de dados: MEDLINE Assunto principal: Fenóis / Dermatopatias / Neoplasias Cutâneas / Flavonoides / Transformação Celular Neoplásica / Crataegus / Edema Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Fenóis / Dermatopatias / Neoplasias Cutâneas / Flavonoides / Transformação Celular Neoplásica / Crataegus / Edema Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article