Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes.
Biochim Biophys Acta
; 1774(6): 756-63, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17513180
ABSTRACT
The glyoxalase (Glx) system is a critical detoxification enzyme system that is widely distributed in prokaryotic and eukaryotic organisms. Glyoxalase I (GlxI), the first enzyme in the system, is a divalent metal-ion dependent lyase (isomerizing), and its homologs have recently been categorized into two metal activation classes which are either Zn2+-dependent or non-Zn2+ dependent (Ni2+-/Co2+-activated). The latter class encompasses enzymes of predominantly bacterial origin. We have identified two genes in Pseudomonas aeruginosa PAO1 encoding glyoxalase I enzymes in addition to the gloA1 sequence recently reported and characterized. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene remarkably encodes a Zn2+-dependent homolog. To our knowledge this is the first report of a eubacterial species with several GlxI encoding genes, and also of an organism possessing GlxI enzymes from both metal activation classes.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Genes Bacterianos
/
Lactoilglutationa Liase
/
Metais
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article