[A practical method of numerical evaluation applicable to miniaturized technics of plaque assay]. / Méthode pratique d'évaluation numérique applicable aux techniques miniaturisées de titrage en plages

ABSTRACT

When one wishes to titrate viruses by counting the number of infective foci on small surfaces of observation, one is rapidly limited by confluences which render counting impossible. Between the use of an all or nothing system which is not accurate and a total field of count, often impossible, there is an intermediate method already described and studied by Tippett, which is very little used in practice. We have performed the calculations again, drawn up reference values and given rules for choice of the field. Two examples show their practical use.