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A specific real-time PCR assay for the detection of Bordetella pertussis.
Vincart, Benoit; De Mendonça, Ricardo; Rottiers, Sylvianne; Vermeulen, Françoise; Struelens, Marc J; Denis, Olivier.
Afiliação
  • Vincart B; Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • De Mendonça R; Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Rottiers S; Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Vermeulen F; Department of Paediatrics, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Struelens MJ; Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Denis O; Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
J Med Microbiol ; 56(Pt 7): 918-920, 2007 Jul.
Article em En | MEDLINE | ID: mdl-17577056
A novel real-time PCR (RT-PCR) assay was developed for detection of Bordetella pertussis in respiratory specimens by targeting the pertactin gene. In vitro evaluation with reference strains and quality control samples showed analytical sensitivity equivalent to and specificity superior to those of PCR assays which target the IS481 element. The pertactin-based RT-PCR assay offers better discrimination between B. pertussis and other Bordetella species than previously described assays.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Bordetella pertussis / Fatores de Virulência de Bordetella / Coqueluche / Reação em Cadeia da Polimerase Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Bordetella pertussis / Fatores de Virulência de Bordetella / Coqueluche / Reação em Cadeia da Polimerase Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article