Increased protein and mRNA expression of endostatin in the ischemic brain tissue of rabbits after middle cerebral artery occlusion.
Neurosci Bull
; 23(1): 35-40, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17592523
ABSTRACT
OBJECTIVE:
To explore the changes of endostatin (a strong anti-angiogenesis factor) and vascular endothelial growth factor (VEGF) in the brain tissues of rabbits following cerebral ischemia induced by middle cerebral artery occlusion (MCAO).METHODS:
Twenty-four New Zealand white rabbits were randomly divided into 5 groups control (n = 5), sham-operation (n = 4), 2-hour ischemia (n = 5), 24-hour ischemia (n = 5), and 48-hour ischemia (n = 5). The expression of VEGF and endostatin were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. In situ hybridization was used to characterize the expression of mRNA for the endostatin.RESULTS:
Both the protein (at least 50%, P < 0.01) and mRNA (at least 70%, P < 0.05) of endostatin increased significantly in the ischemic brain tissues after MCAO compared with the control group. VEGF increased at least 270% in the brain after cerebral ischemia (P < 0.05).CONCLUSION:
Cerebral ischemia leads to an up-regulation of endostatin in the brain, which is not associated with the increase of VEGF in the brain. The increase of endostatin may serve as a deleterious mechanism for ischemic injury through blocking angiogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Regulação para Cima
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Isquemia Encefálica
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Infarto da Artéria Cerebral Média
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Endostatinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article