The impact of process stress on suspended anchorage-dependent mammalian cells as an indicator of likely challenges for regenerative medicines.
Biotechnol Bioeng
; 99(2): 468-74, 2008 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-17626302
ABSTRACT
To quantify the engineering shear constraint on processing, the effect of capillary shear stress (pipe flow) on suspended anchorage-dependent mammalian cells has been investigated. Exposure of cultured rat aortic smooth muscle cells to repeated capillary shear stress (2-120 N m(-2)) causes a decrease in total number of cells, number of intact cells and number of cells able to grow. The optimum wall shear stress for cell survival was found to be 10-50 N m(-2) (flowrate 4-20 mL/min, I.D. 0.45 mm). Cell populations which are able to grow after exposure to shear stress do not exhibit reduced growth rate or altered metabolism.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Órgãos
/
Reologia
/
Células Imobilizadas
/
Miócitos de Músculo Liso
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article