Your browser doesn't support javascript.
loading
Morphological and metabolic changes in the cortex of mice lacking the functional presynaptic active zone protein bassoon: a combined 1H-NMR spectroscopy and histochemical study.
Angenstein, Frank; Hilfert, Liane; Zuschratter, Werner; Altrock, Wilko D; Niessen, Heiko G; Gundelfinger, Eckart D.
Afiliação
  • Angenstein F; Special Laboratory for Non-Invasive Brain Imaging, Leibniz Institute for Neurobiology, Magdeburg, Germany. angenstein@ifn-magdeburg.de
Cereb Cortex ; 18(4): 890-7, 2008 Apr.
Article em En | MEDLINE | ID: mdl-17652465
ABSTRACT
Mice lacking functional presynaptic active zone protein Bassoon are characterized by an enlarged cerebral cortex and an altered cortical activation pattern. This morphological and functional phenotype is associated with defined metabolic distortions as detected by a metabonomic approach using high-field (14.1 T) high-resolution 1H-nuclear magnetic resonance spectroscopy (MRS) in conjunction with statistical pattern recognition. Within the cortex but not in the cerebellum, concentrations of N-acetyl aspartate, glutamine, and glutamate are significantly reduced, whereas the majority of all other detectable low molecular metabolites are unchanged. The reduction of the neuron-specific metabolite N-acetyl aspartate in the cortex coincides with a significant decrease in neuronal density in cortical layer V. Comparing the neuron with glia cell densities across the cortex reveals cortex layer-dependent alterations in the ratio between both cell types. Whereas the ratio shifts significantly toward neurons in the cortical input layers IV, the ratio is reversed in cortical layer V. Consequently, the previously observed altered neuronal activation pattern in the cortex is reflected not only in defined cytoarchitectural anomalies but also in metabolic disturbances in the glutamine-glutamate and N-acetyl aspartate metabolism.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Córtex Cerebral / Ressonância Magnética Nuclear Biomolecular / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Córtex Cerebral / Ressonância Magnética Nuclear Biomolecular / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article