Cloning and functional expression in Saccharomyces cereviae of a K+ transporter, AlHAK, from the graminaceous halophyte, Aeluropus littoralis.
Biotechnol Lett
; 29(12): 1959-63, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-17657411
ABSTRACT
High-affinity K(+) transporters play an important role in K(+) absorption of plants. We isolated a HAK gene from Aeluropus littoralis, a graminaceous halophyte. The amino acid sequence of AlHAK showed high homology with HAK transporters obtained from Oryza sativa (82%) and Hordeum vulgare (82%). When expressed in Saccharomyces cereviae WDelta3, AlHAK performed high-affinity K(+) uptake with a K(m) value of 8 muM, and the growth of transformants was dramatically inhibited by 150 mM Rb(+) and 150 mM Cs(+) but less affected by 300 mM Na(+). AlHAK may thus improve the capacity of plants to maintain a high cytosolic K(+)/Na(+) ratio at high salinity.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Proteínas de Plantas
/
Saccharomyces cerevisiae
/
Poaceae
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article