HPLC method for the determination of nystatin in saliva for application in clinical studies.
J Pharm Biomed Anal
; 45(3): 526-30, 2007 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-17658236
ABSTRACT
An isocratic high-performance liquid chromatographic method was developed, optimized and validated for the determination of nystatin in human saliva (UV and fluorescence detection). A reversed-phase Luna C18 column (25 degrees C), with a mobile phase of MeOH, H2O, and DMF (702010, v/v/v), and a flow-rate of 0.8 ml/min were used. The elution time for nystatin was 5.8+/-0.2 min. Calibration curves in human saliva were linear from 0.78 to 50 microg/ml. Limits of quantification were 0.78 microg/ml and 0.75 microg/ml for UV and fluorescence detection, respectively. The accuracy and precision values of intra- and inter-day variation studies were within acceptable limits, according to FDA guidelines. The described method has proved to be useful to give accurate measurements of nystatin in real samples.
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Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Nistatina
/
Cromatografia Líquida de Alta Pressão
/
Antifúngicos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article