[Study on the enrichment of oleuropein from Olea europaea leaves with macroporous resin D-101].

ABSTRACT

OBJECTIVE: To study the conditions and parameters of the enrichment of oleuropein with macroporous resin. METHODS: Aqueous extract of Olea europaea leaves prepared through microwave extraction was adsorbed directly with macroporous resin D-101 and the impurities such sugar were washed out by water then oleuropein was eluted by 70% ethanol. HPLC was used to determine the content of oleuropein. RESULTS: The contents of oleuropein increased from 5% to 21.6% in the solid, with 88.6% of recovery rate. CONCLUSION: Macroporous resin D-101 fits in purification of water-soluble oleuropein. The process is simple and convenient and can be used for industrial production.