Replication of an RNA ligase ribozyme under alternating temperature condition.
FEBS Lett
; 581(21): 4065-72, 2007 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-17674971
ABSTRACT
Self-replication process of the RNA ligase ribozyme molecules was investigated by using the modified RNA ligase ribozyme under alternating temperature condition that enhances turnover rate of the RNA ligation reaction. In our experiment, the RNA ligase ribozyme system mainly undergoes a cross-catalytic replication process, in which two ribozymes catalyze each other's synthesis from a total of four RNA substrates under alternating temperature condition, resulting in time-dependent accumulation of additional copies of the starting ribozymes in a reaction mixture. The present study demonstrates that cross-catalytic replication in nucleic acids system can be efficiently devised under the alternating temperature condition.
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Base de dados:
MEDLINE
Assunto principal:
RNA Catalítico
/
Modelos Químicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article