A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen.
J Mol Biol
; 372(5): 1137-48, 2007 Oct 05.
Article
em En
| MEDLINE
| ID: mdl-17720188
ABSTRACT
During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human proteins.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Antígeno Nuclear de Célula em Proliferação
/
Proteoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article